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Issue 6, 2015
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New knowledge on the antiglycoxidative mechanism of chlorogenic acid

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Abstract

The role of chlorogenic acid (CGA) in the formation of advanced glycation end-products (AGEs) (glycoxidation reaction) was studied. Model systems composed of bovine serum albumin (BSA) (1 mg mL−1) and methylglyoxal (5 mM) under mimicked physiological conditions (pH 7.4, 37 °C) were used to evaluate the antiglycoxidative effect of CGA (10 mM). The stability of CGA under reaction conditions was assayed by HPLC and MALDI-TOF MS. The glycoxidation reaction was estimated by analysis of free amino groups by the OPA assay, spectral analysis of fluorescent AGEs and total AGEs by ELISA, and colour formation by absorbance at 420 nm. Structural changes in protein were evaluated by analysis of phenol bound to the protein backbone using the Folin reaction, UV-Vis spectral analysis and MALDI-TOF-MS, while changes in protein function were measured by determining the antioxidant capacity using the ABTS radical cation decolourisation assay. CGA was isomerised and oxidised under our experimental conditions. Evidence of binding between BSA and multiple CGA and/or its derivative molecules (isomers and oxidation products) was found. CGA inhibited (p < 0.05) the formation of fluorescent and total AGEs at 72 h of reaction by 91.2 and 69.7%, respectively. The binding of phenols to BSA significantly increased (p < 0.001) its antioxidant capacity. Correlations between free amino group content, phenol bound to protein and antioxidant capacity were found. Results indicate that CGA simultaneously inhibits the formation of potentially harmful compounds (AGEs) and promotes the generation of neoantioxidant structures.

Graphical abstract: New knowledge on the antiglycoxidative mechanism of chlorogenic acid

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Publication details

The article was received on 25 Feb 2015, accepted on 14 May 2015 and first published on 14 May 2015


Article type: Paper
DOI: 10.1039/C5FO00194C
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Citation: Food Funct., 2015,6, 2081-2090

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    New knowledge on the antiglycoxidative mechanism of chlorogenic acid

    B. Fernandez-Gomez, M. Ullate, G. Picariello, P. Ferranti, M. D. Mesa and M. D. del Castillo, Food Funct., 2015, 6, 2081
    DOI: 10.1039/C5FO00194C

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