Jump to main content
Jump to site search

Issue 7, 2015
Previous Article Next Article

Effect of tannic acid–fish scale gelatin hydrolysate hybrid nanoparticles on intestinal barrier function and α-amylase activity

Author affiliations

Abstract

Practical application of tannic acid is limited because it readily binds proteins to form insoluble aggregates. In this study, tannic acid was self-assembled with fish scale gelatin hydrolysates (FSGH) to form stable colloidal complex nanoparticles. The nanoparticles prepared from 4 mg ml−1 tannic acid and 4 mg ml−1 FSGH had a mean particle size of 260.8 ± 3.6 nm, and showed a positive zeta potential (20.4 ± 0.4 mV). The nanoparticles acted as effective nano-biochelators and free radical scavengers because they provided a large number of adsorption sites for interaction with heavy metal ions and scavenging free radicals. The maximum adsorption capacity for Cu2+ ions was 123.5 mg g−1 and EC50 of DPPH radical scavenging activity was 21.6 ± 1.2 μg ml−1. Hydroxyl radical scavenging effects of the nanoparticles were investigated by electron spin resonance spectroscopy. The copper-chelating capacity and free radical scavenging activity of the nanoparticles were associated with their capacity to inhibit Cu2+ ion-induced barrier impairment and hyperpermeability of Caco-2 intestinal epithelial tight junction (TJ). However, α-amylase inhibitory activity of the nanoparticles was significantly lower than that of free tannic acid. The results suggest that the nanoparticles can ameliorate Cu2+ ion induced intestinal epithelial TJ dysfunction without severely inhibiting the activity of the digestive enzymes.

Graphical abstract: Effect of tannic acid–fish scale gelatin hydrolysate hybrid nanoparticles on intestinal barrier function and α-amylase activity

Back to tab navigation

Publication details

The article was received on 07 Nov 2014, accepted on 26 May 2015 and first published on 27 May 2015


Article type: Paper
DOI: 10.1039/C4FO01015A
Author version
available:
Download author version (PDF)
Food Funct., 2015,6, 2283-2292

  •   Request permissions

    Effect of tannic acid–fish scale gelatin hydrolysate hybrid nanoparticles on intestinal barrier function and α-amylase activity

    S. Wu, Y. Ho, S. Jiang and F. Mi, Food Funct., 2015, 6, 2283
    DOI: 10.1039/C4FO01015A

Search articles by author

Spotlight

Advertisements