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Volume 187, 2016
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SERS as a tool for in vitro toxicology

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Measuring markers of stress such as pH and redox potential are important when studying toxicology in in vitro models because they are markers of oxidative stress, apoptosis and viability. While surface enhanced Raman spectroscopy is ideally suited to the measurement of redox potential and pH in live cells, the time-intensive nature and perceived difficulty in signal analysis and interpretation can be a barrier to its broad uptake by the biological community. In this paper we detail the development of signal processing and analysis algorithms that allow SERS spectra to be automatically processed so that the output of the processing is a pH or redox potential value. By automating signal processing we were able to carry out a comparative evaluation of the toxicology of silver and zinc oxide nanoparticles and correlate our findings with qPCR analysis. The combination of these two analytical techniques sheds light on the differences in toxicology between these two materials from the perspective of oxidative stress.

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The article was received on 09 Dec 2015, accepted on 22 Jan 2016 and first published on 28 Jan 2016

Article type: Paper
DOI: 10.1039/C5FD00216H
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Faraday Discuss., 2016,187, 501-520

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    SERS as a tool for in vitro toxicology

    K. M. Fisher, J. A. McLeish, L. E. Jamieson, J. Jiang, J. R. Hopgood, S. McLaughlin, K. Donaldson and C. J. Campbell, Faraday Discuss., 2016, 187, 501
    DOI: 10.1039/C5FD00216H

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