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Issue 47, 2015
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Photo-triggered fluorescent labelling of recombinant proteins in live cells

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Abstract

A method to photo-chemically trigger fluorescent labelling of proteins in live cells is developed. The approach is based on photo-caged split-intein mediated conditional protein trans-splicing reaction and enabled background-free fluorescent labelling of target proteins with the necessary spatiotemporal control.

Graphical abstract: Photo-triggered fluorescent labelling of recombinant proteins in live cells

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Publication details

The article was received on 05 Feb 2015, accepted on 05 May 2015 and first published on 15 May 2015


Article type: Communication
DOI: 10.1039/C5CC01067E
Citation: Chem. Commun., 2015,51, 9670-9673

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    Photo-triggered fluorescent labelling of recombinant proteins in live cells

    D. Jung, K. Sato, K. Min, A. Shigenaga, J. Jung, A. Otaka and Y. Kwon, Chem. Commun., 2015, 51, 9670
    DOI: 10.1039/C5CC01067E

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