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Issue 47, 2015
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A general method for synthesizing enzyme–polymer conjugates in reverse emulsions using Pluronic as a reactive surfactant

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Abstract

Using aldehyde-functionalized Pluronic as the reactive surfactant, enzyme–Pluronic conjugates with sizes ranging from nanometers to micrometers were synthesized in reverse emulsions. Compared with the direct conjugation in aqueous solution, this method gave an increased conjugation efficiency and well-controlled size of the conjugates. The versatility of this method was validated using horseradish peroxidase (HRP), Candida rugosa lipase (CRL) and Candida antarctica lipase B (CALB). The resulting enzyme–Pluronic conjugates showed greatly enhanced apparent activity compared to free enzymes in organic media.

Graphical abstract: A general method for synthesizing enzyme–polymer conjugates in reverse emulsions using Pluronic as a reactive surfactant

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Publication details

The article was received on 02 Mar 2015, accepted on 05 May 2015 and first published on 05 May 2015


Article type: Communication
DOI: 10.1039/C5CC01776A
Citation: Chem. Commun., 2015,51, 9674-9677

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    A general method for synthesizing enzyme–polymer conjugates in reverse emulsions using Pluronic as a reactive surfactant

    X. Wu, J. Ge, J. Zhu, Y. Zhang, Y. Yong and Z. Liu, Chem. Commun., 2015, 51, 9674
    DOI: 10.1039/C5CC01776A

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