A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin αvβ3in vitro

Zhiyuan Hu; Rory L. Arrowsmith; James A. Tyson; Vincenzo Mirabello; Haobo Ge; Ian M. Eggleston; Stanley W. Botchway; G. Dan Pantos; Sofia I. Pascu

doi:10.1039/C4CC08265F

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A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin α_vβ₃in vitro†

Zhiyuan Hu,*^ab Rory L. Arrowsmith,^b James A. Tyson,^b Vincenzo Mirabello,^b Haobo Ge,^b Ian M. Eggleston,^c Stanley W. Botchway,^d G. Dan Pantos*^b and Sofia I. Pascu*^b

Author affiliations

* Corresponding authors

^a State Key Lab of Heavy Oil Processing, China University of Petroleum-Beijing, No. 18 Fuxue Road, Beijing, China
E-mail: zh209@cup.edu.cn

^b Department of Chemistry, University of Bath, Claverton Down, UK
E-mail: s.pascu@bath.ac.uk, g.d.pantos@bath.ac.uk

^c Department of Pharmacy and Pharmacology, University of Bath, Claverton Down, UK

^d Central Laser Facility, Rutherford Appleton Laboratory, Research Complex at Harwell, STFC, Didcot, UK

Abstract

We have developed a fluorescent peptide conjugate (TrpNDIRGDfK) based on the coupling of cyclo(RGDfK) to a new tryptophan-tagged amino acid naphthalenediimide (TrpNDI). Confocal fluorescence microscopy coupled with fluorescence lifetime imaging (FLIM) mapping, single and two-photon fluorescence excitation, lifetime components and corresponding decay profiles were used as parameters able to investigate qualitatively the cellular behavior regarding the molecular environment and biolocalisation of TrpNDI and TrpNDI–RGDfK in cancer cells.

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Article information

DOI: https://doi.org/10.1039/C4CC08265F
Article type: Communication
Submitted: 19 Oct 2014
Accepted: 18 Dec 2014
First published: 16 Jan 2015

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Chem. Commun., 2015,51, 6901-6904

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A fluorescent Arg–Gly–Asp (RGD) peptide–naphthalenediimide (NDI) conjugate for imaging integrin α_vβ₃in vitro

Z. Hu, R. L. Arrowsmith, J. A. Tyson, V. Mirabello, H. Ge, I. M. Eggleston, S. W. Botchway, G. Dan Pantos and S. I. Pascu, Chem. Commun., 2015, 51, 6901 DOI: 10.1039/C4CC08265F

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