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Issue 57, 2014
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Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

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Abstract

A triazole linkage is formed in RNA by untemplated strain-promoted or CuAAC chemical ligation of 3′-azide and 5′-cyclooctyne oligonucleotides under denaturing conditions. Reverse transcriptase reads through these artificial linkages with omission of one nucleotide. These surprising results have implications for RNA isolation, amplification, sequencing and a variety of biological applications.

Graphical abstract: Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

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Supplementary files

Article information


Submitted
23 Apr 2014
Accepted
22 May 2014
First published
29 May 2014

Chem. Commun., 2014,50, 7597-7600
Article type
Communication
Author version available

Reverse transcription through a bulky triazole linkage in RNA: implications for RNA sequencing

X. Chen, A. H. El-Sagheer and T. Brown, Chem. Commun., 2014, 50, 7597 DOI: 10.1039/C4CC03027C

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