Direct determination of phenolic acids and hydroxymethylfurfural in wines elaborated in Vale do São Francisco region-Brazil by HPLC DAD
Abstract
The present paper describes a direct method for the determination of gallic acid, hydroxymethylfurfural, vanillic, caffeic, p-coumaric, ferulic, caftaric, cis-coutaric, fertaric and trans-coutaric acids in Brazilian red wines by direct injection employing reverse phase high performance liquid chromatography (RP HPLC DAD). The method was performed using a sample volume of 10 μL and the separation process was established employing a monolithic column. The method was applied for analysis of red wine samples collected from the São Francisco region, Bahia State, Brazil. Ten wine samples were analysed and the contents of caftaric acid varied from 13.28 to 46.83 mg L−1, cis-coutaric acid from 1.01 to 2.13 mg L−1, trans-coutaric acid from 2.13 to 17.56 mg L−1, fertaric acid from 1.11 to 2.43 mg L−1, ferulic acid from 0.31 to 3.97 mg L−1, hydroxymethylfurfural from 0.59 to 8.83 mg L−1, vanillic acid from 2.90 to 11.57 mg L−1, caffeic acid from 4.20 to 14.20 mg L−1, p-coumaric acid from 0.26 to 12.46 mg L−1 and ferulic acid from 0.31 to 3.97 mg L−1.