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Issue 4, 2017
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Simultaneous analysis of kasugamycin and validamycin-A in fruits and vegetables using liquid chromatography-tandem mass spectrometry and consecutive solid-phase extraction

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Abstract

A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method for simultaneous determination of two hydrophilic aminoglycosides, kasugamycin and validamycin-A, in a variety of matrices (apples, cabbage, cucumbers, lettuce, tomatoes, and eggplant) has been developed. The proposed method provides sufficient sensitivity and excellent chromatographic performance using a special ReproSil 100 C18 column with 0.1% formic acid as the mobile phase additive. Accurate mass information for both analytes was obtained based on time-of-flight (TOF) mass spectrometry. The methanol/water (70 : 30, v/v) with pH adjusted to 5.5 was selected as the extraction solvent. The combination of hydrophilic–hydrophobic-balanced (HLB) cartridges with strong cation exchange SCX cartridges was used to purify the extracts with strongly polar compounds and satisfactory results were obtained. The consecutive solid-phase extraction minimized signal suppression/enhancement which led to matrix effects in the range of −12.6 to 6.11% only. Recovery experiments were performed for both compounds at three levels (50, 100, and 500 μg kg−1) in six different matrices and yielded good recoveries ranging from 81.7% to 108% with relative standard deviation values below 13%. The limits of quantification (LOQs) for kasugamycin and validamycin-A were 4.1 μg kg−1 and 1.0 μg kg−1, respectively.

Graphical abstract: Simultaneous analysis of kasugamycin and validamycin-A in fruits and vegetables using liquid chromatography-tandem mass spectrometry and consecutive solid-phase extraction

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Supplementary files

Article information


Submitted
25 Oct 2016
Accepted
23 Dec 2016
First published
23 Dec 2016

Anal. Methods, 2017,9, 634-642
Article type
Paper

Simultaneous analysis of kasugamycin and validamycin-A in fruits and vegetables using liquid chromatography-tandem mass spectrometry and consecutive solid-phase extraction

C. Wang, H. Li, N. Wang, H. Li, L. Fang, Z. Dong, H. Du, S. Guan, Q. Zhu, Z. Chen and G. Yang, Anal. Methods, 2017, 9, 634
DOI: 10.1039/C6AY02921C

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