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Issue 12, 2014
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Structure-specific hapten design for the screening of highly sensitive and specific monoclonal antibody to salbutamol

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Abstract

Salbutamol (SAL) is a short-acting β2-adrenergic receptor agonist (β-agonist) forbidden in livestock production. To develop a more sensitive and specific detection method for SAL, a new hapten was synthesized, keeping the structure of benzene ring side of SAL while changing the tert-butyl group structure that SAL shares with other β-agonists. Ten mice were immunized with a SAL hapten–keyhole limpet hemocyanin (KLH) conjugate, and cells were selected within a urine sample at the cell fusion stage. The best monoclonal antibody (Mab) had an IC50 value of 0.31 ng mL−1 in PBST buffer and an IC50 value of 0.19 ng mL−1 in a swine urine sample diluted five times (with a lower B0 value), and had no cross-reactivity with clenbuterol and other β-agonists. These results show that the hapten design was successful and that the Mab can potentially be used in highly sensitive immunoassays for specifically monitoring the illegal use of SAL in real samples.

Graphical abstract: Structure-specific hapten design for the screening of highly sensitive and specific monoclonal antibody to salbutamol

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Article information


Submitted
08 Jan 2014
Accepted
13 Mar 2014
First published
17 Mar 2014

Anal. Methods, 2014,6, 4228-4233
Article type
Paper
Author version available

Structure-specific hapten design for the screening of highly sensitive and specific monoclonal antibody to salbutamol

L. Liu, H. Kuang, C. Peng, L. Wang and C. Xu, Anal. Methods, 2014, 6, 4228
DOI: 10.1039/C4AY00062E

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