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Issue 12, 2014
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Metabolic profiling of Piper species by direct analysis using real time mass spectrometry combined with principal component analysis

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Abstract

Piper nigrum (black pepper), Piper longum (Indian tipali) and Piper chaba (Bangla tipali) are widely used medicinal herbs in traditional Indian systems of medicine. P. longum as well as P. chaba are considered to be inferior substitutes of P. nigrum. A rapid analytical method for metabolic profiling was developed to generate chemical fingerprints as an alternative means to the only available morphological basis for the identification of these species. Direct analysis using real time mass spectrometry was applied to generate the chemical fingerprints of various parts viz. fruit, leaf and root of these Piper species followed by multivariate analysis for discrimination. Principal component analysis of DARTMS data served as a reliable method for species identification. Phytochemical analysis showed the presence mainly of alkaloids in various parts of Piper species. The results showed that different Piper species could be successfully differentiated using DARTMS fingerprinting together with principal component analysis and this method is promising for the rapid identification and quality control of Piper species.

Graphical abstract: Metabolic profiling of Piper species by direct analysis using real time mass spectrometry combined with principal component analysis

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Supplementary files

Article information


Submitted
28 Jan 2014
Accepted
23 Mar 2014
First published
12 May 2014

Anal. Methods, 2014,6, 4234-4239
Article type
Paper

Metabolic profiling of Piper species by direct analysis using real time mass spectrometry combined with principal component analysis

P. Chandra, V. Bajpai, M. Srivastva, K. B. R. Kumar and B. Kumar, Anal. Methods, 2014, 6, 4234
DOI: 10.1039/C4AY00246F

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