A separation-free method for simultaneous determination of sucrose and sunset yellow in different abundance by integrating RBI and TLdetectors

Abstract

A novel method for simultaneous determination of sucrose and sunset yellow in retail soft drink without separation by combination of retro-reflected beam interference-based refractive index (RBI) and thermal lens (TL) detections was developed. The method proposed here successfully realized individual measurements of sucrose and sunset yellow in one single sample, while their concentrations were thousands of times different in the sample. Under optimized conditions, a separation-free process was carried out so that sucrose and sunset yellow were quantified at the same time. Limits of detection (LODs) of sucrose and sunset yellow were 0. 21 mg ml⁻¹ and 0.23 μg ml⁻¹ and the relative standard deviation (RSDs) were 3.9% and 3.07%. The developed method was successfully applied for the simultaneous analysis of sucrose and sunset yellow in retail soft drink. The soft drink containing 123.6 ± 2.6 mg ml⁻¹ sucrose and 46.6 ± 1.8 μg ml⁻¹ sunset yellow have been simultaneously measured without any separation procedure.