Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 3, 2011
Previous Article Next Article

A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes

Author affiliations

Abstract

We report the fabrication of an amperometric whole-cell herbicide biosensor based on magnetic retention of living cells functionalised with magnetic nanoparticles (MNPs) on the surface of a screen-printed electrode. We demonstrate that Chlorella pyrenoidosa microalgae cells coated with biocompatible MNPs and retained on the electrode with a permanent magnet act as a sensing element for the fast detection of herbicides. The magnetic functionalisation does not affect the viability and photosynthesis activity-mediated triazine herbicide recognition in microalgae. The current of ferricyanide ion was recorded during alternating illumination periods and biosensor fabricated was used to detect atrazine (from 0.9 to 74 µM) and propazine (from 0.6 to 120 µM) (the limits of detection 0.7 and 0.4 µM, respectively). We believe that the methodology presented here can be widely used in fabrication of a number of whole cell biosensors since it allows for efficient and reversible cells immobilisation and does not affect the cellular metabolism.

Graphical abstract: A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes

Back to tab navigation

Supplementary files

Article information


Submitted
18 Oct 2010
Accepted
02 Jan 2011
First published
24 Jan 2011

Anal. Methods, 2011,3, 509-513
Article type
Communication

A whole-cell amperometric herbicide biosensor based on magnetically functionalised microalgae and screen-printed electrodes

A. I. Zamaleeva, I. R. Sharipova, R. V. Shamagsumova, A. N. Ivanov, G. A. Evtugyn, D. G. Ishmuchametova and R. F. Fakhrullin, Anal. Methods, 2011, 3, 509
DOI: 10.1039/C0AY00627K

Social activity

Search articles by author

Spotlight

Advertisements