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Issue 22, 2014
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Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

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Abstract

Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.

Graphical abstract: Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

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Supplementary files

Article information


Submitted
22 Jun 2014
Accepted
21 Aug 2014
First published
22 Aug 2014

This article is Open Access

Analyst, 2014,139, 5709-5717
Article type
Paper
Author version available

Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

A. M. Schmidt, S. R. Fagerer, K. Jefimovs, F. Buettner, C. Marro, E. C. Siringil, K. L. Boehlen, M. Pabst and A. J. Ibáñez, Analyst, 2014, 139, 5709
DOI: 10.1039/C4AN01119H

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