Issue 11, 2024

Embryonic development through in vitro fertilization using high-quality bovine sperm separated in a biomimetic cervix environment

Abstract

This study is the first to identify bovine blastocysts through in vitro fertilization (IVF) of matured oocytes with a large quantity of high-quality sperm separated from a biomimetic cervix environment. We obtained high-quality sperm in large quantities using an IVF sperm sorting chip (SSC), which could mimic the viscous environment of the bovine cervix during ovulation and facilitates isolation of progressively motile sperm from semen. The viscous environment-on-a-chip was realized by formulating and implementing polyvinylpyrrolidone (PVP)-based solutions for the SSC medium. Sperm separated from the IVF-SSC containing PVP 1.5% showed high motility, normal morphology and high DNA integrity. As a result of IVF, a higher rate of hatching blastocysts, which is the pre-implantation stage, were observed, compared to the conventional swim-up method. Our results may significantly contribute to improving livestock with superior male and female genetic traits, thus overcoming the limitation of artificial insemination based on the superior genetic traits of existing males.

Graphical abstract: Embryonic development through in vitro fertilization using high-quality bovine sperm separated in a biomimetic cervix environment

Supplementary files

Article information

Article type
Communication
Submitted
31 Jan 2024
Accepted
23 Apr 2024
First published
01 May 2024

Analyst, 2024,149, 3078-3084

Embryonic development through in vitro fertilization using high-quality bovine sperm separated in a biomimetic cervix environment

D. G. Byeun, D. Kim, J. H. Park, M. Lee and J. K. Choi, Analyst, 2024, 149, 3078 DOI: 10.1039/D4AN00166D

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