Issue 22, 2023, Issue in Progress

Point-of-care paper-based analytical device for potentiometric detection of myoglobin as a cardiovascular disease biomarker

Abstract

One of the cardiac biomarkers, myoglobin (Mb), is important in the rapid identification of cardio-vascular disorders. Therefore, point-of-care monitoring is essential. Pursuing this goal, a robust, reliable, and affordable paper-based analytical apparatus for potentiometric sensing has been developed and characterized. The molecular imprint technique was used to create a customized biomimetic antibody for myoglobin (Mb) on the surface of carboxylated multiwalled carbon nanotubes (MWCNT–COOH). This was accomplished by attaching Mb to carboxylated MWCNTs' surfaces and then filling the empty spaces through the mild polymerization of acrylamide in N,N-methylenebisacrylamide and ammonium persulphate. The modification of the MWCNTs' surface was verified by SEM and FTIR analysis. A hydrophobic paper substrate coated with fluorinated alkyl silane (CF3(CF2)7CH2CH2SiCl3, CF10) has been coupled with a printed all–solid–state Ag/AgCl reference electrode. The presented sensors showed a linear range of 5.0 × 10−8 to 1.0 × 10−4 M with a potentiometric slope of −57.1 ± 0.3 mV decade−1 (R2 = 0.9998) and a detection limit of 28 nM at pH 4. Compared to creatinine, sucrose, fructose, galactose, sodium glutamate, thiamine, alanine, ammonium, uric acid, albumin, glutamine, guanine, troponine T, and glucose, the sensor showed good selectivity for Mb. It demonstrated a good recovery for the detection of Mb in several fake serum samples (93.0–103.3%), with an average relative standard deviation of 4.5%. The current approach might be viewed as a potentially fruitful analytical tool for obtaining disposable, cost-effective paper-based potentiometric sensing devices. These types of analytical devices can be potentially manufacturable at large scales in clinical analysis.

Graphical abstract: Point-of-care paper-based analytical device for potentiometric detection of myoglobin as a cardiovascular disease biomarker

Article information

Article type
Paper
Submitted
10 Apr 2023
Accepted
01 May 2023
First published
18 May 2023
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2023,13, 15199-15207

Point-of-care paper-based analytical device for potentiometric detection of myoglobin as a cardiovascular disease biomarker

A. A. Almehizia, A. M. Naglah, L. S. Alrasheed, M. G. Alanazi, A. E. E. Amr and A. H. Kamel, RSC Adv., 2023, 13, 15199 DOI: 10.1039/D3RA02375C

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