CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor

Ling Wan; Jianfeng Ma; Jiasheng Yi; Yan Dong; Renjie Niu; Yan Su; Qian Li; Dan Zhu; Jie Chao; Shao Su; Chunhai Fan; Lianhui Wang; Ying Wan

doi:10.1039/D2CC01155G

CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor†

Ling Wan,‡^a Jianfeng Ma,‡^a Jiasheng Yi,^a Yan Dong,^a Renjie Niu,^a Yan Su,^b Qian Li,

^c Dan Zhu,^a Jie Chao,^a Shao Su,*^a Chunhai Fan,

^c Lianhui Wang

^a and Ying Wan

*^b

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* Corresponding authors

^a State Key Laboratory of Organic Electronics and Information Displays & Jiangsu Key Laboratory for Biosensors, Institute of Advanced Materials (IAM), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Nanjing 210023, China
E-mail: iamssu@njupt.edu.cn

^b School of Mechanical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China
E-mail: wanying@njust.edu.cn

^c School of Chemistry and Chemical Engineering, Frontiers Science Center for Transformative Molecules and National Center for Translational Medicine, Shanghai Jiao Tong University, Shanghai 200240, China

Abstract

Rapid pathogen screening holds the key against certain viral infections, especially in an overwhelming pandemic. Herein, a CRISPR-empowered electrochemical biosensor was designed for the ultrasensitive detection of the avian influenza A (H7N9) virus gene sequence. Combining the CRISPR/Cas system, a signal-amplification strategy and a high-conductivity sensing substrate, the developed biosensor showed an ultrawide dynamic range, an ultralow detection limit, and excellent selectivity for H7N9 detection, providing a potential sensing platform for the simple, fast, sensitive, and on-site detection of infectious diseases.

Chemical Communications

CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor†

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CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor

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