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Issue 5, 2021
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Efficient bioconversion of high-concentration d-fructose into d-mannose by a novel N-acyl-d-glucosamine 2-epimerase from Thermobifida halotolerans

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Abstract

The synthesis of mannose is of great value because it is the only glyconutrient used for clinical applications. Here, we performed the enzymatic bioconversion of D-fructose to D-mannose using a recombinant thermostable N-acyl-D-glucosamine 2-epimerase (ThMI) from a Thermobifida halotolerans engineered strain. The enzymatic bioconversion produced 180 g L−1D-mannose from high-concentration D-fructose (500 g L−1) in 1 h by recombinant ThMI, resulting in a maximum bioconversion ratio of 35.8%. The recombinant resting cells at a density of 10 OD600 produced 157 g L−1D-mannose from 500 g L−1D-fructose in 1 h. These results suggest that the novel biocatalyst system developed in this study can be applied for large-scale industrial production of D-mannose.

Graphical abstract: Efficient bioconversion of high-concentration d-fructose into d-mannose by a novel N-acyl-d-glucosamine 2-epimerase from Thermobifida halotolerans

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Article information


Submitted
30 Sep 2020
Accepted
28 Dec 2020
First published
29 Dec 2020

Catal. Sci. Technol., 2021,11, 1922-1930
Article type
Paper

Efficient bioconversion of high-concentration D-fructose into D-mannose by a novel N-acyl-D-glucosamine 2-epimerase from Thermobifida halotolerans

P. Jin, Y. Wang, Z. Liang, M. Yuan, H. Li and Q. Du, Catal. Sci. Technol., 2021, 11, 1922
DOI: 10.1039/D0CY01915A

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