Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application

Zhanying Chu; Manman Zhu; Weibing Zhang; Yang Zhao; Xiaoyun Gong; You Jiang; Liqing Wu; Rui Zhai; Xinhua Dai; Xiang Fang

doi:10.1039/D1CC04467B

Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application†

Zhanying Chu,

^a Manman Zhu,^a Weibing Zhang,

^b Yang Zhao,^a Xiaoyun Gong,^a You Jiang,^a Liqing Wu,^a Rui Zhai,*^a Xinhua Dai*^a and Xiang Fang*^a

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* Corresponding authors

^a Technology Innovation Center of Mass Spectrometry for State Market Regulation, Center for Advanced Measurement Science, National Institute of Metrology, Beijing, 100029, P. R. China
E-mail: zhairui@nim.ac.cn, daixh@nim.ac.cn, fangxiang@nim.ac.cn

^b Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai, P. R. China

Abstract

A facile, controllable and environmentally friendly method for fabricating a novel polysaccharide–silica composite stationary phase (SiO₂@(HA-CS)₁₂) was developed in this report. Two natural polysaccharides (hyaluronan acid and chitosan) were controllably coated on the silica surface using a layer-by-layer assembly technique, and then the polysaccharide shell was chemically cross-linked to improve the stability. The column efficiency of the SiO₂@(HA-CS)₁₂ column reached 74 000 plates per m in HILIC mode and 20 100 plates per m in IEC mode, which indicates great potential for separating polar and charged samples.

Chemical Communications

Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application†

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Layer-by-layer coating and chemical cross-linking of multilayer polysaccharides on silica for mixed-mode HPLC application

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