Specific enrichment and glycosylation discrepancy profiling of cellular exosomes using a dual-affinity probe

Abstract

A hydrophilic probe is employed to enrich exosomes from three kinds of cancer cells by TiO₂–phosphate interaction and exosomal glycoproteins by hydrophilic interaction in succession. The probe performs efficiently in both the enrichment processes. And the analytical results confirm that unique exosomal glycoproteins can distinguish parent exosomes from others.