Issue 30, 2021

Promiscuous dye binding by a light-up aptamer: application for label-free multi-wavelength biosensing

Abstract

Light-up DNA aptamers are promising label-free signal-transducers for biosensing applications due to their high chemical stability and low synthetic cost. Herein, we demonstrate that a dapoxyl DNA aptamer DAP-10–42 can be converted into a sensor generating a fluorescence signal at different wavelengths in the range of 500–660 nm depending on the dye that is present. This results from the discovered promiscuity of DAP-10–42 in binding fluorogenic dyes including arylmethane dyes. We have designed a split DAP-10–42 aptasensor for the detection of a katG gene fragment from Mycobacterium tuberculosis with a point mutation causing isoniazid resistance. Efficient interrogation of the gene fragment after nucleic acid sequence-based amplification (NASBA) is achieved directly in a protein-containing NASBA sample. This report lays a foundation for the application of the DAP-10–42 aptamer as a versatile sensing platform.

Graphical abstract: Promiscuous dye binding by a light-up aptamer: application for label-free multi-wavelength biosensing

Supplementary files

Article information

Article type
Communication
Submitted
01 Feb 2021
Accepted
07 Mar 2021
First published
08 Mar 2021

Chem. Commun., 2021,57, 3672-3675

Promiscuous dye binding by a light-up aptamer: application for label-free multi-wavelength biosensing

R. P. Connelly, P. F. Madalozzo, J. E. Mordeson, A. D. Pratt and Y. V. Gerasimova, Chem. Commun., 2021, 57, 3672 DOI: 10.1039/D1CC00594D

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