Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation

Joongoo Lee; Rafael Torres; Do Soon Kim; Michelle Byrom; Andrew D. Ellington; Michael C. Jewett

doi:10.1039/D0CC02121K

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Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation†

Joongoo Lee,

^a Rafael Torres,^a Do Soon Kim,

^a Michelle Byrom,^b Andrew D. Ellington^b and Michael C. Jewett

*^a

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* Corresponding authors

^a Department of Chemical and Biological Engineering and Center for Synthetic Biology, Northwestern University, Evanston, IL 60208, USA
E-mail: m-jewett@northwestern.edu

^b Department of Chemistry and Biochemistry, Institute for Cellular and Molecular Biology, University of Texas at Austin, Austin, 78712 TX, USA

Abstract

We demonstrate in vitro incorporation of cyclic β-amino acids into peptides by the ribosome through genetic code reprogramming. Further, we show that incorporation efficiency can be increased through the addition of elongation factor P.

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DOI: https://doi.org/10.1039/D0CC02121K
Article type: Communication
Submitted: 22 Mar 2020
Accepted: 24 Apr 2020
First published: 24 Apr 2020

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Chem. Commun., 2020,56, 5597-5600

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Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation

J. Lee, R. Torres, D. S. Kim, M. Byrom, A. D. Ellington and M. C. Jewett, Chem. Commun., 2020, 56, 5597 DOI: 10.1039/D0CC02121K

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Chemical Communications

Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation†

Abstract

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Ribosomal incorporation of cyclic β-amino acids into peptides using in vitro translation

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