Issue 66, 2020
From the journal:

Chemical Communications

Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown†

Beob Soo Kim, ORCID logo a   Mitsuru Naito, ORCID logo b   Rimpei Kamegawa,a   Hyun Jin Kim, ORCID logo b   Ryo Iizuka,c   Takashi Funatsu,c   Shingo Ueno,d   Takanori Ichiki,ad   Akihiro Kishimurae  and  Kanjiro Miyata ORCID logo *a  

* Corresponding authors

a Department of Materials Engineering, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
E-mail: miyata@bmw.t.u-tokyo.ac.jp

b Center for Disease Biology and Integrative Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

c Graduate School of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

d Innovation Center of NanoMedicine, Kawasaki Institute of Inductrial Promotion, 3-25-14 Tonomachi, Kawasaki-ku, Kawasaki 210-0821, Japan

e Center for Molecular Systems, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan

Abstract

A photo-responsive nanovesicle is fabricated by polyion complex (PIC) formation between poly(ethylene glycol) (PEG)-block-polypeptides and photo-reactive oligodeoxynucleotides (PROs)/anti-sense oligonucleotides (ASOs). The ultraviolet (UV) light triggers reversible crosslinking between PROs and ASOs in the vesicular membrane, providing the nanovesicle with switchable stability under physiological conditions. The resulting nanovesicle allows efficient cellular internalization, leading to significant UV-triggered gene knockdown in cultured cells.

Graphical abstract: Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

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Article information

DOI
https://doi.org/10.1039/D0CC01750G
Article type
Communication
Submitted
06 Mar 2020
Accepted
30 Jun 2020
First published
17 Jul 2020
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2020,56, 9477-9480

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Photo-reactive oligodeoxynucleotide-embedded nanovesicles (PROsomes) with switchable stability for efficient cellular uptake and gene knockdown

B. S. Kim, M. Naito, R. Kamegawa, H. J. Kim, R. Iizuka, T. Funatsu, S. Ueno, T. Ichiki, A. Kishimura and K. Miyata, Chem. Commun., 2020, 56, 9477 DOI: 10.1039/D0CC01750G

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