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A Versatile Bacterial Membrane-Binding Chimeric Peptide with Enhanced Photodynamic Antimicrobial Activity

Abstract

Photodynamic therapy (PDT) has become an effective antibiosis method for overcoming antibiotic resistance. In this study, we developed a versatile bacterial membrane-binding chimeric peptide PpIX-[PEG8-(KLAKLAK)2]2 (denoted as PPK) by conjugating a photosensitizer protoporphyrin IX (PpIX) with the antimicrobial peptide (KLAKLAK)2 (KLA) for effective photodynamic inactivation of bacteria. The chimeric peptide PPK with positively charged property and α-helical conformation could rapidly bind to microbial cells through electrostatic interaction and membrane insertion. Moreover, PPK could disrupt bacterial membrane and further elicit lipid bilayer leakage to kill bacteria by toxic reactive oxygen species (ROS) generated by PpIX under 660 nm light. In vitro experiments demonstrated that cationic PPK possessed excellent antimicrobial activity against both Gram-positive bacteria Staphylococcus aureus (S. aureus) and Gram-negative bacteria Escherichia coli (E. coli). Afterward, PPK also exhibited perfect therapeutic effects on S. aureus-infected mice without any systemic side effect. This chimeric peptide PPK will find great potential for photodynamic antibiosis.

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Publication details

The article was received on 23 Nov 2018, accepted on 07 Jan 2019 and first published on 08 Jan 2019


Article type: Paper
DOI: 10.1039/C8TB03094D
Citation: J. Mater. Chem. B, 2019, Accepted Manuscript
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    A Versatile Bacterial Membrane-Binding Chimeric Peptide with Enhanced Photodynamic Antimicrobial Activity

    A. Zhang, W. Wu, C. Zhang, Q. Wang, Z. Zhuang, H. Cheng and X. Zhang, J. Mater. Chem. B, 2019, Accepted Manuscript , DOI: 10.1039/C8TB03094D

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