Issue 47, 2019, Issue in Progress

Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells

Abstract

Chicken embryo fibroblast DF-1 cells are increasingly being used in the production of avian virus vaccines. However, the relatively low proliferative capacity does not meet the requirements of industrial production. In this study, we attempted to improve the proliferative capacity of DF-1 cells. The results of intracellular metabolomics showed that 28 types of metabolites could play roles in DF-1 cell growth based on the variance and timing analysis of intracellular metabolites from DF-1 cells grown in two media with distinct growth difference, DMEM/F12 (1 : 1) and DMEM. By examining the differences in the components in the two media, DOE was used to screen and optimize the growth medium for DF-1 cells. The maximum cell density was 40.72% higher, and the infectious bursal disease virus (IBDV) titer was 2.68 times higher, in the optimized medium than in the control. This study proposes a complete solution from metabolomics to media optimization.

Graphical abstract: Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells

Supplementary files

Article information

Article type
Paper
Submitted
09 Jul 2019
Accepted
15 Aug 2019
First published
30 Aug 2019
This article is Open Access
Creative Commons BY-NC license

RSC Adv., 2019,9, 27369-27377

Medium optimization based on comparative metabolomic analysis of chicken embryo fibroblast DF-1 cells

J. Lin, X. Yi and Y. Zhuang, RSC Adv., 2019, 9, 27369 DOI: 10.1039/C9RA05128G

This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. You can use material from this article in other publications, without requesting further permission from the RSC, provided that the correct acknowledgement is given and it is not used for commercial purposes.

To request permission to reproduce material from this article in a commercial publication, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party commercial publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements