Jump to main content
Jump to site search


X-ray tomography shows the varying three-dimensional morphology of gold nanoaggregates in the cellular ultrastructure

Author affiliations

Abstract

The processing of nanoparticles inside eukaryotic cells is a key step in many wanted and unwanted nano-bio-interactions. In order to understand the effects and functions of the intracellular aggregates that are formed, their properties and their interaction with the biological matrix must be characterized. High quality synchrotron soft X-ray tomography (SXT) data were obtained from cells containing gold nanoparticles that are commonly applied as tools for optical probing or drug delivery. 3D volume rendering of both cellular organelles and the nanoparticle aggregates of different sizes in the intact cells of two cell lines reveals variation in localization, size, shape and density of the intracellular gold nanoaggregates. The dependence of such variation on incubation time and cell type, as well as on the influence of pre-aggregation of primary nanoparticles is shown. The SXT results provide a detailed picture of intracellular aggregation and will improve the design of safe and efficient nanoparticle platforms for biomedical use.

Graphical abstract: X-ray tomography shows the varying three-dimensional morphology of gold nanoaggregates in the cellular ultrastructure

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Mar 2019, accepted on 08 Jun 2019 and first published on 13 Jun 2019


Article type: Paper
DOI: 10.1039/C9NA00198K
Nanoscale Adv., 2019, Advance Article
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    X-ray tomography shows the varying three-dimensional morphology of gold nanoaggregates in the cellular ultrastructure

    D. Drescher, T. Büchner, P. Guttmann, S. Werner, G. Schneider and J. Kneipp, Nanoscale Adv., 2019, Advance Article , DOI: 10.1039/C9NA00198K

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements