Control of CRISPR-Cas9 with small molecule-activated allosteric aptamer regulating sgRNAs

Bingqian Lin; Yuan An; Lingyan Meng; Huimin Zhang; Jia Song; Zhi Zhu; Wen Liu; Yanling Song; Chaoyong Yang

doi:10.1039/C9CC05531B

Control of CRISPR-Cas9 with small molecule-activated allosteric aptamer regulating sgRNAs†

Bingqian Lin,‡^a Yuan An,‡^a Lingyan Meng,^a Huimin Zhang,^b Jia Song,^b Zhi Zhu,^a Wen Liu,*^c Yanling Song

*^ab and Chaoyong Yang

*^ab

Author affiliations

* Corresponding authors

^a MOE Key Laboratory of Spectrochemical Analysis & Instrumentation, Collaborative Innovation Center of Chemistry for Energy Materials, Key Laboratory for Chemical Biology of Fujian Province, State Key Laboratory of Physical Chemistry of Solid Surfaces, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
E-mail: ylsong@fzu.edu.cn, cyyang@xmu.edu.cn

^b Institute of Molecular Medicine, Renji Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China

^c School of Pharmaceutical Sciences, Fujian Provincial Key Laboratory of Innovative Drug Target Research, Xiamen University, Xiang’an South Road, Xiamen, Fujian 361102, China
E-mail: w2liu@xmu.edu.cn

Abstract

The CRISPR-Cas9 system enables facile and efficient genome engineering in living cells and organisms. Small molecule control of CRISPR-Cas9 would allow precise temporal control in applications of the technology. In this work, we developed [s with combining low line] mall [m with combining low line] olecule-activated allosteric [a with combining low line] ptamer [r with combining low line] egula [t with combining low line] ing (SMART)-sgRNAs as general strategies to control programmable genome editing. With SMART-sgRNAs, temporal control of the CRISPR-Cas9 system against different targets can be achieved, which should facilitate its application in various fields, such as biomedical genome editing, drug screening and chromosome imaging.

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DOI: https://doi.org/10.1039/C9CC05531B
Article type: Communication
Submitted: 17 Jul 2019
Accepted: 09 Sep 2019
First published: 09 Sep 2019

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Chem. Commun., 2019,55, 12223-12226

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Control of CRISPR-Cas9 with small molecule-activated allosteric aptamer regulating sgRNAs

B. Lin, Y. An, L. Meng, H. Zhang, J. Song, Z. Zhu, W. Liu, Y. Song and C. Yang, Chem. Commun., 2019, 55, 12223 DOI: 10.1039/C9CC05531B

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Chemical Communications

Control of CRISPR-Cas9 with small molecule-activated allosteric aptamer regulating sgRNAs†

Abstract

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Control of CRISPR-Cas9 with small molecule-activated allosteric aptamer regulating sgRNAs

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