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Issue 5, 2018
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Electron beam lithography of poly(glycidol) nanogels for immobilization of a three-enzyme cascade

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Abstract

Surface immobilization of multiple enzymes plays an increasingly important role in biosensor development. Traditional nanofabrication methods require multiple fabrication steps and complex syntheses which often result in limited enzyme density, modest spatial control and decreased enzyme activity over time and repeated uses, particularly when extensive synthetic modifications are applied. We employed electron beam lithography to crosslink poly(glycidol) with pre-loaded enzyme nanogels, which contain crosslinked poly(glycidol), on a silicon surface to enhance the stability and robustness of synergistic enzymes. As proof-of-concept, we immobilized three enzymes β-galactosidase, glucose oxidase, and horseradish peroxidase with and without nanogel pre-entrapment, and bioactivity was investigated via colorimetric determination with lactose substrate and o-phenylenediamine indicator. The resulting enzyme nanogel device exhibited 71% retention of bioactivity after 30 days and 5-fold higher chromogenic output compared with 32% from free enzyme devices.

Graphical abstract: Electron beam lithography of poly(glycidol) nanogels for immobilization of a three-enzyme cascade

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Publication details

The article was received on 14 Nov 2017, accepted on 10 Jan 2018 and first published on 17 Jan 2018


Article type: Paper
DOI: 10.1039/C7PY01904A
Citation: Polym. Chem., 2018,9, 637-645
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    Electron beam lithography of poly(glycidol) nanogels for immobilization of a three-enzyme cascade

    J. N. Lockhart, A. B. Hmelo and E. Harth, Polym. Chem., 2018, 9, 637
    DOI: 10.1039/C7PY01904A

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