Jump to main content
Jump to site search

Issue 24, 2018
Previous Article Next Article

Functional TCR T cell screening using single-cell droplet microfluidics

Author affiliations

Abstract

Adoptive T cell transfer, in particular TCR T cell therapy, holds great promise for cancer immunotherapy with encouraging clinical results. However, finding the right TCR T cell clone is a tedious, time-consuming, and costly process. Thus, there is a critical need for single cell technologies to conduct fast and multiplexed functional analyses followed by recovery of the clone of interest. Here, we use droplet microfluidics for functional screening and real-time monitoring of single TCR T cell activation upon recognition of target tumor cells. Notably, our platform includes a tracking system for each clone as well as a sorting procedure with 100% specificity validated by downstream single cell reverse-transcription PCR and sequencing of TCR chains. Our TCR screening prototype will facilitate immunotherapeutic screening and development of T cell therapies.

Graphical abstract: Functional TCR T cell screening using single-cell droplet microfluidics

Back to tab navigation

Supplementary files

Article information


Submitted
07 Aug 2018
Accepted
26 Oct 2018
First published
06 Nov 2018

Lab Chip, 2018,18, 3733-3749
Article type
Paper

Functional TCR T cell screening using single-cell droplet microfluidics

A. I. Segaliny, G. Li, L. Kong, C. Ren, X. Chen, J. K. Wang, D. Baltimore, G. Wu and W. Zhao, Lab Chip, 2018, 18, 3733
DOI: 10.1039/C8LC00818C

Social activity

Search articles by author

Spotlight

Advertisements