Novel fluorescent antibody probes, which show antigen-dependent fluorescence responses, were developed by selective N-terminal fluorescent labeling of IgG monoclonal antibodies using a reductive alkylation. Several kinds of IgG against tag peptides and small molecules were successfully utilized to detect antigens in a rapid and quantitative manner.
K. Fukunaga, T. Watanabe, D. Novitasari, H. Ohashi, R. Abe and T. Hohsaka, Chem. Commun., 2018, 54, 12734 DOI: 10.1039/C8CC07827K
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