Issue 81, 2018
From the journal:

Chemical Communications

Live imaging of cell membrane-localized MT1-MMP activity on a microfluidic chip†

Nan Li,‡a   Weifei Zhang,‡a   Ling Lin, ORCID logo *b   Ziyi He,a   Mashooq Khan ORCID logo a  and  Jin-Ming Lin ORCID logo *a  

* Corresponding authors

a Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, MOE Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, China
E-mail: jmlin@mail.tsinghua.edu.cn

b CAS Key Laboratory of Standardization and Measurement for Nanotechnology, CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, Beijing, China
E-mail: linling@nanoctr.cn

Abstract

We designed an enzyme-activatable probe for real time in situ tracking of MT1-MMP activity. The MT1-MMP responses of endothelial cells were investigated under the regulation of shear stress and biochemical factor on a microfluidic chip. This strategy can be beneficial to evaluate membrane protein responses to external stimuli and to engineer cells for basic and clinical research.

Graphical abstract: Live imaging of cell membrane-localized MT1-MMP activity on a microfluidic chip

About
Cited by
Related
Download options Please wait...

Supplementary files

Article information

DOI
https://doi.org/10.1039/C8CC07117A
Article type
Communication
Submitted
01 Sep 2018
Accepted
17 Sep 2018
First published
17 Sep 2018

Chem. Commun., 2018,54, 11435-11438

Permissions

Request permissions

Live imaging of cell membrane-localized MT1-MMP activity on a microfluidic chip

N. Li, W. Zhang, L. Lin, Z. He, M. Khan and J. Lin, Chem. Commun., 2018, 54, 11435 DOI: 10.1039/C8CC07117A

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements