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Issue 25, 2016
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Inhibitor-assisted synthesis of silica-core microbeads with pepsin-imprinted nanoshells

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A novel approach for molecularly imprinting proteins, i.e. inhibitor-assisted imprinting, onto silica microspheres is discussed, which provides advanced functional materials addressing prevalent challenges in the field of protein purification and isolation from biotechnologically relevant media. Pepstatin-assisted surface-imprinted core–shell microbeads for the acidic protease pepsin were synthesized serving as selective sorbent materials for solid phase extraction (SPE) applications. The inorganic core, i.e. amino-functionalized silica spheres (AFSS), is prepared by the co-condensation of tetraethylorthosilicate (TEOS) and (3-aminopropyl) trimethoxysilane (APTMS) in water-in-oil (W/O) emulsion, which is then reacted with pepstatin, a selective inhibitor of pepsin, onto the surface of the AFSS via an amide bond. 3-Aminophenylboronic acid (APBA) serves as the functional monomer for establishing nanothin imprinted polymer films, i.e. poly(3-aminophenylboronic acid) (pAPBA) at the surface of the pepstatin-immobilized AFSS via oxidation by ammonium persulfate in aqueous solution in the presence (molecularly imprinted polymer, MIP) and absence (non-imprinted polymer; NIP) of pepsin. Thus obtained core–shell microbeads are packaged into SPE cartridges for evaluating the selectivity for pepsin. Each individual synthesis step is thoroughly characterized using x-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and BET methods. Finally, the imprinted core–shell microbeads indeed provide specific binding.

Graphical abstract: Inhibitor-assisted synthesis of silica-core microbeads with pepsin-imprinted nanoshells

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Publication details

The article was received on 18 Jan 2016, accepted on 09 May 2016 and first published on 10 May 2016

Article type: Paper
DOI: 10.1039/C6TB00147E
J. Mater. Chem. B, 2016,4, 4462-4469

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    Inhibitor-assisted synthesis of silica-core microbeads with pepsin-imprinted nanoshells

    M. Dinc, H. Basan, T. Diemant, R. J. Behm, M. Lindén and B. Mizaikoff, J. Mater. Chem. B, 2016, 4, 4462
    DOI: 10.1039/C6TB00147E

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