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Issue 28, 2016
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A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

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Abstract

Anoectochilus formosanus is a famous traditional Taiwanese folk medicine. Kinsenoside (1), one of the major active components in A. formosanus, exhibits hepatoprotective, antihyperliposis and antiflammatory activities. In this study, a proton nuclear magnetic resonance (1H NMR) method was developed for rapid quantitative analysis of several bioactive major constituents, including kinsenoside (1), 3-(R)-3-β-D-gluco-pyranosyloxy-4-hydroxybutanoic acid (2), 1-O-isopropyl-β-D-gluco-pyranoside (3), 4-(β-D-gluco-pyranosyloxy)benzyl alcohol (4) and benzoic acid (5) in plant samples and commercial products of A. formosanus. Interferences were removed by a simple Diaion HP-20 column chromatography. In the 1H NMR spectrum, the signals of compounds 1 (δ 2.88), 2 (δ 2.47), 3 (δ 1.12), 4 (δ 7.05) and 5 (δ 7.84) were selected and integrated as the target peaks, separated well from other signals in D2O. The developed 1H NMR method provides a rapid and simple quantitative method without any derivatization, pH control or addition of NMR shift reagent, and the analytical solvent system is very safe, convenient and simple.

Graphical abstract: A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

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Publication details

The article was received on 08 Mar 2016, accepted on 12 Jun 2016 and first published on 15 Jun 2016


Article type: Paper
DOI: 10.1039/C6AY00670A
Anal. Methods, 2016,8, 5645-5650

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    A rapid quantitative 1H NMR analysis of kinsenoside and other bioactive principles from Anoectochilus formosanus

    L. Hsieh, H. Chan, H. Hung, C. Kuo, J. Wu, I. Sun, P. Kuo and T. Wu, Anal. Methods, 2016, 8, 5645
    DOI: 10.1039/C6AY00670A

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