Jump to main content
Jump to site search

Issue 7, 2015
Previous Article Next Article

Simple electrochemical sensing of attomolar proteins using fabricated complexes with enhanced surface binding avidity

Author affiliations

Abstract

Various strategies have been proposed for the detection of disease protein biomarkers; however, most methods are too expensive, cumbersome or limited in sensitivity for clinical use. Here, we report that a fabricated complex can be used as a powerful tool to detect trace proteins in complex samples. In this strategy, a DNA–protein complex that comprises of one target molecule and two or more deoxyribozyme-containing probes can exhibit autonomous cleavage behavior on the surface of the substrate DNA modified electrode. In the meantime, the complex can remove the cleaved DNA fragment from the electrode surface by taking advantage of the proximity effect. The proposed approach allows one-step and highly sensitive detection of a variety of targets based on the changes of the direct electrochemical readout. Moreover, this method may also have considerable advantages over the commonly reported DNA amplification-assisted immunoassays, particularly in terms of assay simplicity and cost, which may hold great potential for application in resource-constrained regions.

Graphical abstract: Simple electrochemical sensing of attomolar proteins using fabricated complexes with enhanced surface binding avidity

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Mar 2015, accepted on 04 May 2015 and first published on 05 May 2015


Article type: Edge Article
DOI: 10.1039/C5SC00891C
Citation: Chem. Sci., 2015,6, 4311-4317
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Simple electrochemical sensing of attomolar proteins using fabricated complexes with enhanced surface binding avidity

    C. Li, X. Li, L. Wei, M. Liu, Y. Chen and G. Li, Chem. Sci., 2015, 6, 4311
    DOI: 10.1039/C5SC00891C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements