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Issue 89, 2015
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Effects of terminal group and chain length on temperature-responsive chromatography utilizing poly(N-isopropylacrylamide) synthesized via RAFT polymerization

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Abstract

Poly(N-isopropylacrylamide) with two chain lengths (MW: ca. 5000, and ca. 20 000 g mol−1) was synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization, using 2-dodecylsulfanylthiocarbonylsulfanyl-2-methyl propionic acid as the chain transfer agent. The derivatization of terminal dodecyl groups to maleimide groups was performed through the reduction of terminal trithiocarbonate moieties. These polymers were grafted onto aminopropyl silica using an activated ester–amine coupling method. Five types of polymer modified silica beads were prepared, i.e., long chain length, short chain length, and mixed chain length functionalized with a dodecyl terminal group, and long chain length and short chain length with a maleimide terminal group. The surface properties of the modified polymer silica beads were evaluated by employing them as the stationary phase in HPLC setups and examining the temperature-dependent elution profiles of five steroids. The retention factor of steroids became smaller when the terminal dodecyl group was substituted with a maleimide group. A hydrophobic dodecyl moiety on the outermost surface strongly affected the retention of steroids, and the retention factor of steroids on short chains was larger than that on long chains below the lower critical solution temperature. These results indicate that the chain length and terminal functional group on the outermost surface of the polymer have a critical effect on the characteristics of polymer modified silica bead interfaces.

Graphical abstract: Effects of terminal group and chain length on temperature-responsive chromatography utilizing poly(N-isopropylacrylamide) synthesized via RAFT polymerization

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Supplementary files

Article information


Submitted
08 Aug 2015
Accepted
24 Aug 2015
First published
24 Aug 2015

This article is Open Access

RSC Adv., 2015,5, 73217-73224
Article type
Paper
Author version available

Effects of terminal group and chain length on temperature-responsive chromatography utilizing poly(N-isopropylacrylamide) synthesized via RAFT polymerization

Y. Hiruta, Y. Nagumo, A. Miki, T. Okano and H. Kanazawa, RSC Adv., 2015, 5, 73217
DOI: 10.1039/C5RA15906G

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