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Issue 81, 2015
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Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation

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Abstract

Thermoresponsive hydrophobic copolymer, poly(N-isopropylacrylamide-co-n-butyl methacrylate) [P(IPAAm-co-BMA)], brushes were prepared on porous monolithic silica rods through surface-initiated atom transfer radical polymerization (ATRP). The monolithic silica surface was modified with the ATRP-initiator [(chloromethyl)phenylethyl]trimethoxysilane (CPTMS) via silanization performed by flowing a CPTMS toluene solution into the monolithic silica rod column. ATRP was performed by flowing a reaction solution containing IPAAm and BMA monomers, and ATRP catalyst CuCl/CuCl2/tris[2-(N,N-dimethylamino)ethyl]amine in 2-propanol into the monolithic silica. Characterization by CHN elemental analysis, X-ray photoelectron spectroscopy (XPS), and gel permeation chromatography revealed that dense P(IPAAm-co-BMA) brushes were formed on the monolith surface. Chromatographic analysis using benzoic acids and insulin peptides indicated that the brush-modified monolithic silica was able to separate these analytes with high resolution and in short analysis times, owing to the effective interaction between the modified polymer brushes and the analyte. These results indicated that the present P(IPAAm-co-BMA) brush modified monolithic silica is an effective separation tool for analyzing bio-related molecules.

Graphical abstract: Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation

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Supplementary files

Article information


Submitted
10 Jun 2015
Accepted
22 Jul 2015
First published
23 Jul 2015

RSC Adv., 2015,5, 66155-66167
Article type
Paper

Thermoresponsive hydrophobic copolymer brushes modified porous monolithic silica for high-resolution bioseparation

K. Nagase, J. Kobayashi, A. Kikuchi, Y. Akiyama, H. Kanazawa and T. Okano, RSC Adv., 2015, 5, 66155
DOI: 10.1039/C5RA11038F

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