Issue 81, 2015

Fabrication and evaluation of protein imprinted polymer based on magnetic halloysite nanotubes

Abstract

A novel protein imprinted polymer, which combined the surface imprinting technology and magnetic halloysite nanotubes (MHNTs), was prepared for selective separation of a template protein. In this work, MHNTs were synthesized through the coprecipitation method based on encapsulation of Fe3O4 nanoparticles in halloysite nanotubes. The surface of the MHNTs was modified with vinyl groups through reaction with 3-(methacryloyloxy)propyl trimethoxysilane. Subsequently, the modified MHNTs were used as a support, N-isopropylacrylamide and methacrylic acid as bifunctional monomers, N,N′-methylene bisacrylamide as the crosslinking agent, and ammonium persulphate and N,N,N′,N′-tetramethylethylenediamine as initiators. The resulting polymer was named MHNTs@BSA-MIP, which was characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, X-ray diffraction, and vibrating sample magnetometry. The MHNTs@BSA-MIP exhibited high adsorption (48.4 mg g−1), good selectivity, rapid kinetic binding (45 min), fast magnetic separation (10 s), and favorable reproducibility (relative standard deviation <8% for batch-to-batch evaluation). The prepared MHNTs@BSA-MIP is suitable for separation and provides a significant reference for other proteins in proteomics.

Graphical abstract: Fabrication and evaluation of protein imprinted polymer based on magnetic halloysite nanotubes

Article information

Article type
Paper
Submitted
24 May 2015
Accepted
28 Jul 2015
First published
28 Jul 2015

RSC Adv., 2015,5, 66147-66154

Author version available

Fabrication and evaluation of protein imprinted polymer based on magnetic halloysite nanotubes

X. Zhu, H. Li, H. Zhou and S. Zhong, RSC Adv., 2015, 5, 66147 DOI: 10.1039/C5RA09740A

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