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Issue 16, 2015
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Structure, antimicrobial activity, albumin- and DNA-binding of manganese(ii)–sparfloxacinato complexes

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Abstract

Manganese(II) complexes with the quinolone antimicrobial agent sparfloxacin (Hsf) in the absence or presence of the nitrogen-donor heterocyclic ligands 1,10-phenanthroline (phen), 2,2′-bipyridine (bipy), 2,2′-bipyridylamine (bipyam) or pyridine (py) were synthesized and characterized with diverse physicochemical and spectroscopic techniques. The crystal structure of complex [Mn(sf)2(phen)]·4MeOH was determined by X-ray crystallography. In the resultant complexes, the deprotonated sparfloxacinato ligands are bidentately bound to manganese(II) through the pyridone oxygen and a carboxylato oxygen. The antimicrobial activity of the complexes was tested against four different microorganisms (Escherichia coli, Xanthomonas campestris, Staphylococcus aureus and Bacillus subtilis) and was found to be similar to or higher than free Hsf. The binding of the complexes to calf-thymus DNA (CT DNA) was monitored by UV spectroscopy and DNA viscosity measurements, which indicated intercalation as the most possible mode, and the DNA-binding constants of the complexes were calculated. The ability of the complexes to displace ethidium bromide (EB) from the EB–DNA complex was also investigated. Fluorescence emission spectroscopy was used to evaluate the interaction of the complexes with human or bovine serum albumin proteins revealing their binding with relatively high binding constant values.

Graphical abstract: Structure, antimicrobial activity, albumin- and DNA-binding of manganese(ii)–sparfloxacinato complexes

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Supplementary files

Article information


Submitted
02 Oct 2014
Accepted
06 Jan 2015
First published
08 Jan 2015

This article is Open Access

RSC Adv., 2015,5, 11861-11872
Article type
Paper
Author version available

Structure, antimicrobial activity, albumin- and DNA-binding of manganese(II)–sparfloxacinato complexes

M. Zampakou, S. Balala, F. Perdih, S. Kalogiannis, I. Turel and G. Psomas, RSC Adv., 2015, 5, 11861
DOI: 10.1039/C4RA11682H

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