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Issue 24, 2015
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Separation of cancer cells from white blood cells by pinched flow fractionation

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Abstract

In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell types. Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. CTCs are rare cells in blood, but they are important for the understanding of metastasis. There is therefore a high interest in developing a method for the enrichment of CTCs from blood samples, which also enables further analysis of the separated cells. The separation is challenged by the size overlap between cancer cells and the 106 times more abundant WBCs. The size overlap prevents high efficiency separation, however we demonstrate that cell deformability can be exploited in PFF devices to gain higher efficiencies than expected from the size distribution of the cells.

Graphical abstract: Separation of cancer cells from white blood cells by pinched flow fractionation

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Supplementary files

Article information


Submitted
24 Aug 2015
Accepted
20 Oct 2015
First published
21 Oct 2015

This article is Open Access

Lab Chip, 2015,15, 4598-4606
Article type
Paper
Author version available

Separation of cancer cells from white blood cells by pinched flow fractionation

M. Pødenphant, N. Ashley, K. Koprowska, K. U. Mir, M. Zalkovskij, B. Bilenberg, W. Bodmer, A. Kristensen and R. Marie, Lab Chip, 2015, 15, 4598
DOI: 10.1039/C5LC01014D

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