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Issue 20, 2015
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Simultaneous binding of a cyclophane and classical intercalators to DNA: observation of FRET-mediated white light emission

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Abstract

DNA-assisted Förster resonance energy transfer (FRET) between an anthracene-based cyclophane (CP) and mono- and bis-intercalators such as propidium iodide (PI) and ethidium homodimer-1 (EHD), respectively, has been studied using various photophysical and biophysical techniques. The cyclophane and PI exhibited simultaneous binding to DNA at all concentrations studied and showed DNA-assisted FRET from the excimer of cyclophane with a FRET efficiency of ca. 71%. On the other hand, the bis-intercalator EHD, only at lower concentrations (<3 μM), can act as an acceptor for the energy transfer process with a lower efficiency of ca. 44%. At higher concentrations (>15 μM), EHD, on account of its higher binding affinity, displaces cyclophane from the DNA scaffold. Employing the ternary system comprising of the cyclophane, DNA and PI and fine-tuning the concentrations of the components in a molar ratio of 1 : 0.75 : 0.05 (CP : DNA : PI) we have demonstrated white light emission with CIE coordinates (0.35, 0.37).

Graphical abstract: Simultaneous binding of a cyclophane and classical intercalators to DNA: observation of FRET-mediated white light emission

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Supplementary files

Article information


Submitted
14 Jan 2015
Accepted
20 Apr 2015
First published
20 Apr 2015

Phys. Chem. Chem. Phys., 2015,17, 13495-13500
Article type
Paper
Author version available

Simultaneous binding of a cyclophane and classical intercalators to DNA: observation of FRET-mediated white light emission

K. S. Sanju, S. Thurakkal, P. P. Neelakandan, J. Joseph and D. Ramaiah, Phys. Chem. Chem. Phys., 2015, 17, 13495
DOI: 10.1039/C5CP00208G

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