Issue 73, 2015
From the journal:

Chemical Communications

Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification†

Yan Zhang,‡a   Wen-jing Xu,‡ab   Ya-ping Zeng‡a  and  Chun-yang Zhang*ac  

* Corresponding authors

a Single-Molecule Detection and Imaging Laboratory, Key Lab of Health Informatics of Chinese Academy of Sciences, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China

b Shenzhen College of Advanced Technology, University of Chinese Academy of Sciences, Shenzhen 518055, China

c College of Chemistry, Chemical Engineering and Materials Science, Collaborative Innovation Center of Functionalized Probes for Chemical Imaging in Universities of Shandong, Key Laboratory of Molecular and Nano Probes, Ministry of Education, Shandong Provincial Key Laboratory of Clean Production of Fine Chemicals, Shandong Normal University, Jinan 250014, China
E-mail: cyzhang@sdnu.edu.cn
Fax: +86-0531-82615258
Tel: +86-0531-86182538

Abstract

We develop a sensitive fluorescence method for DNA methyltransferase (MTase) assay based on T7 RNA polymerase-mediated transcription amplification and duplex-specific nuclease (DSN)-assisted cyclic signal amplification. This method exhibits excellent specificity and high sensitivity with a detection limit of 0.015 U mL−1, and it may be further applied for the screening of antimicrobial drugs.

Graphical abstract: Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification

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Article information

DOI
https://doi.org/10.1039/C5CC05922D
Article type
Communication
Submitted
16 Jul 2015
Accepted
31 Jul 2015
First published
31 Jul 2015

Chem. Commun., 2015,51, 13968-13971

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Sensitive detection of DNA methyltransferase activity by transcription-mediated duplex-specific nuclease-assisted cyclic signal amplification

Y. Zhang, W. Xu, Y. Zeng and C. Zhang, Chem. Commun., 2015, 51, 13968 DOI: 10.1039/C5CC05922D

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