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Issue 13, 2015
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A high-throughput screening method for determining the substrate scope of nitrilases

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Abstract

Nitrile compounds are intermediates in the synthesis of pharmaceuticals such as atorvastatin. We have developed a chromogenic reagent to screen for nitrilase activity as an alternative to Nessler's reagent. It produces a semi-quantifiable blue colour and hydrolysis of 38 nitrile substrates by 23 nitrilases as cell-free extracts has been shown.

Graphical abstract: A high-throughput screening method for determining the substrate scope of nitrilases

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Supplementary files

Article information


Submitted
01 Aug 2014
Accepted
19 Dec 2014
First published
22 Dec 2014

This article is Open Access

Chem. Commun., 2015,51, 2660-2662
Article type
Communication
Author version available

A high-throughput screening method for determining the substrate scope of nitrilases

G. W. Black, N. L. Brown, J. J. B. Perry, P. D. Randall, G. Turnbull and M. Zhang, Chem. Commun., 2015, 51, 2660
DOI: 10.1039/C4CC06021K

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