Issue 36, 2014

Developmental and cartilaginous effects of protein-coated SiO2 nanoparticle corona complexes on zebrafish larvae

Abstract

Protein-coated SiO2 nanoparticle (SNP) corona complexes of different sizes can be selected for various applications, and whether such materials have different degrees of toxicity is becoming a critical question. As the substance that is richest in proteins, bovine serum albumin (BSA-V), is chosen to coat the two types of SNP to obtain SNP corona complexes with different diameters and surface charges, as well as alleviating agglomeration. Toxicity tests were performed in vivo using zebrafish as a platform. Hatch rates, mortalities, malformations and alcian blue-stained cartilaginous deformities were recorded using a stereomicroscope. The results verified that coating SNPs with BSA-V is a suitable method to control diameters and surface charges. The hatch rate results illustrated the concentration-dependent effect of the complex. The mortality results were increased from 54 hour post fertilization (hpf) to 120 hpf, explained by the change in LC50 values. The malformation incidence, average toxicity score of the malformations, and the vertebral column of the zebrafish further supported the idea that 50 nm NP corona complexes have more toxicity. The developmental stages of the zebrafish at 54 hpf is a novel and important time point for evaluating the toxicity of the complex. The results showed that the same protein-coated NP corona complexes had different surface characters and toxicity extents. The 50 nm NP corona complexes are more toxic than the 15 nm NP corona complexes during developmental stages. Meanwhile the cartilaginous toxicity of the vertebral columns provided further explanation.

Graphical abstract: Developmental and cartilaginous effects of protein-coated SiO2 nanoparticle corona complexes on zebrafish larvae

Supplementary files

Article information

Article type
Paper
Submitted
09 Oct 2013
Accepted
21 Feb 2014
First published
26 Feb 2014

RSC Adv., 2014,4, 18541-18548

Developmental and cartilaginous effects of protein-coated SiO2 nanoparticle corona complexes on zebrafish larvae

Y. Wang, X. Li, J. Xue, Y. Zhang and X. Feng, RSC Adv., 2014, 4, 18541 DOI: 10.1039/C3RA45667F

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements