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Issue 20, 2014
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A new paper-based platform technology for point-of-care diagnostics

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Currently, the Lateral flow Immunoassays (LFIAs) are not able to perform complex multi-step immunodetection tests because of their inability to introduce multiple reagents in a controlled manner to the detection area autonomously. In this research, a point-of-care (POC) paper-based lateral flow immunosensor was developed incorporating a novel microfluidic valve technology. Layers of paper and tape were used to create a three-dimensional structure to form the fluidic network. Unlike the existing LFIAs, multiple directional valves are embedded in the test strip layers to control the order and the timing of mixing for the sample and multiple reagents. In this paper, we report a four-valve device which autonomously directs three different fluids to flow sequentially over the detection area. As proof of concept, a three-step alkaline phosphatase based Enzyme-Linked ImmunoSorbent Assay (ELISA) protocol with Rabbit IgG as the model analyte was conducted to prove the suitability of the device for immunoassays. The detection limit of about 4.8 fm was obtained.

Graphical abstract: A new paper-based platform technology for point-of-care diagnostics

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Publication details

The article was received on 07 Jul 2014, accepted on 15 Aug 2014 and first published on 15 Aug 2014

Article type: Paper
DOI: 10.1039/C4LC00786G
Lab Chip, 2014,14, 4042-4049

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    A new paper-based platform technology for point-of-care diagnostics

    R. Gerbers, W. Foellscher, H. Chen, C. Anagnostopoulos and M. Faghri, Lab Chip, 2014, 14, 4042
    DOI: 10.1039/C4LC00786G

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