Issue 97, 2014

A target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors

Abstract

We develop a new method for ultrasensitive detection of folate receptors (FRs) using a target-triggered isothermally exponential amplification reaction (EXPAR)-based DNAzyme biosensor. This method exhibits excellent specificity and high sensitivity with a detection limit as low as 0.23 fM and a large dynamic range of 6 orders of magnitude from 1 fM to 1 nM. It might be further applied for the detection of various small molecule-binding proteins by simply changing the linked small molecule moiety of the hairpin probes.

Graphical abstract: A target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors

Supplementary files

Article information

Article type
Communication
Submitted
12 Sep 2014
Accepted
14 Oct 2014
First published
14 Oct 2014

Chem. Commun., 2014,50, 15393-15396

A target-triggered exponential amplification-based DNAzyme biosensor for ultrasensitive detection of folate receptors

L. Wang, Y. Zhang and C. Zhang, Chem. Commun., 2014, 50, 15393 DOI: 10.1039/C4CC07230H

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