Issue 96, 2014

Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR

Abstract

The progress of a polymerase chain reaction (PCR) was sensitively monitored based on the increase in fluorescence of N,N′-bis(3-aminopropyl)-2,7-diamino-1,8-naphthyridine, which was covalently anchored on the cytosine bulge directly neighbouring the 5′-T_G-3′/5′-CCA-3′ sequence in the hairpin tag at the 5′ end of the PCR primer.

Graphical abstract: Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR

Supplementary files

Article information

Article type
Communication
Submitted
28 Aug 2014
Accepted
08 Oct 2014
First published
10 Oct 2014

Chem. Commun., 2014,50, 15195-15198

Author version available

Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR

F. Takei, X. Chen, G. Yu, T. Shibata, C. Dohno and K. Nakatani, Chem. Commun., 2014, 50, 15195 DOI: 10.1039/C4CC06780K

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