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Issue 19, 2014
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Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin) against Asian longhorned beetle

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Abstract

Azadirachtins are natural triterpenoid compounds derived from Neem tree extracts with potential for use as systemic insecticides against invasive wood-boring insect pests. In this work, a sensitive and selective analytical method has been developed for the simultaneous determination of azadirachtin A and azadirachtin B (3-tigloylazadirachtol) in foliage and twigs of various tree species. Samples were mixed with C18 and primary-secondary amine (PSA), and extracted with acetonitrile. Then, an aliquot of the raw extract was 10-fold diluted with water and directly analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The method was validated in foliage and twig matrices of four different tree species (London Plane Tree, Red/Freemani Maple, Norway Maple and Sugar Maple) that are known hosts of the exotic invasive insect pest – Asian Longhorn Beetle (ALB). Analytical results for replicate (N = 5) samples, fortified at 0.01, 0.1 and 1 mg kg−1, showed good recoveries (86–119%) and precision (<20% RSD). The methodology was successfully applied to the analysis of 200 samples taken from a field experiment designed to investigate uptake, translocation and expression of azadirachtins in representative high-value urban trees followed by stem injection with TreeAzin™.

Graphical abstract: Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin™) against Asian longhorned beetle

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Article information


Submitted
01 Jul 2014
Accepted
31 Jul 2014
First published
01 Aug 2014

Anal. Methods, 2014,6, 8063-8071
Article type
Technical Note
Author version available

Application of liquid chromatography/mass spectrometry in assessment of potential use of azadirachtins (TreeAzin) against Asian longhorned beetle

E. Beltrán, M. Ibáñez, E. Gracia-Lor, J. V. Sancho, F. Hernández and D. G. Thompson, Anal. Methods, 2014, 6, 8063
DOI: 10.1039/C4AY01553C

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