Jump to main content
Jump to site search

Issue 13, 2014
Previous Article Next Article

UPLC-Q-TOF/MS characterization, HPLC fingerprint analysis and species differentiation for quality control of Nigella glandulifera Freyn et Sint seeds and Nigella sativa L. seeds

Author affiliations

Abstract

Nigella glandulifera Freyn et Sint seeds (NG) as well as Nigella sativa L. seeds (NS) have been employed as folk remedies for centuries. However, little work has been done on their quality control. In order to assess the quality and guarantee therapeutic effects, the chemical constituents of the two herbs were extensively characterized by ultra-performance liquid chromatography coupled with electrospray time-of-flight mass spectrometry (UPLC-Q-TOF/MS), and a simple, practical, and reliable HPLC fingerprinting method was established and applied to determination of 20 batches of samples. With their chromatographic fingerprints, the two species were successfully differentiated by pattern recognition methods, namely, principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA). Results from this study show that the proposed analytical methods can be applied to the quality control of the seeds and have high potential to ensure their safety and efficacy.

Graphical abstract: UPLC-Q-TOF/MS characterization, HPLC fingerprint analysis and species differentiation for quality control of Nigella glandulifera Freyn et Sint seeds and Nigella sativa L. seeds

Back to tab navigation

Supplementary files

Publication details

The article was received on 29 Mar 2014, accepted on 18 Apr 2014 and first published on 22 Apr 2014


Article type: Paper
DOI: 10.1039/C4AY00775A
Anal. Methods, 2014,6, 4845-4852

  •   Request permissions

    UPLC-Q-TOF/MS characterization, HPLC fingerprint analysis and species differentiation for quality control of Nigella glandulifera Freyn et Sint seeds and Nigella sativa L. seeds

    Q. Yun, Q. Liu, C. He, X. Ma, X. Gao, A. Talbi and J. Zhou, Anal. Methods, 2014, 6, 4845
    DOI: 10.1039/C4AY00775A

Search articles by author

Spotlight

Advertisements