Issue 11, 2014
From the journal:

Analyst

A novel microfluidic system for the rapid analysis of protein thermal stability†

Xin Yang,a   Jia Liu,a   Ye Lei Xie,b   Yang Wang,b   Hong Ying,ac   Qiong Wu,a   Wei Huanga  and  Gareth Jenkins*ab  

* Corresponding authors

a Jiangsu-Singapore Joint Research Center for Organic/Bio-Electronics & Information Displays and Institute of Advanced Materials (IAM), Nanjing Tech University, 30 South Puzhu Road, Nanjing 211816, P. R. China
E-mail: iamxyang@njtech.edu.cn
Tel: +86 83587982

b Key Laboratory for Organic Electronics & Information Displays (KLOEID), Nanjing University of Posts and Telecommunications, 9 Wenyuan Road, Yadong, Xincheng District, Nanjing 210046, P. R. China
E-mail: iamgjenkins@njupt.edu.cn
Tel: +86 2585866332

c Nanjing Entry-Exit Inspection And Quarantine Bureau, 1 Guojian Road, General Avenue, Jiangning District, Nanjing 211106, P. R. China
E-mail: hongy@jsciq.gov.cn
Fax: +86 2552396011
Tel: +86 2552396011

Abstract

We describe a simple microfluidic device for the rapid analysis of protein thermal stability using a novel imaging method. The change in UV absorption upon thermal denaturation or aggregation of proteins is used to get a spatial image of proteins' folding or aggregation state along a linear temperature gradient.

Graphical abstract: A novel microfluidic system for the rapid analysis of protein thermal stability

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Article information

DOI
https://doi.org/10.1039/C4AN00173G
Article type
Communication
Submitted
27 Jan 2014
Accepted
02 Apr 2014
First published
02 Apr 2014

Analyst, 2014,139, 2683-2686

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A novel microfluidic system for the rapid analysis of protein thermal stability

X. Yang, J. Liu, Y. L. Xie, Y. Wang, H. Ying, Q. Wu, W. Huang and G. Jenkins, Analyst, 2014, 139, 2683 DOI: 10.1039/C4AN00173G

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