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Issue 11, 2013
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The role of transcription in heterologous expression of polyketides in bacterial hosts

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Abstract

Heterologous expression of biosynthetic pathways is an indispensable tool in the discovery, production, engineering, and characterization of bacterial polyketides and the complex enzymology involved in their biosynthesis. Ensuring transcription of polyketide biosynthetic gene clusters in heterologous hosts is a pressing problem. This review evaluates the two strategies used to ensure transcription. The first is a promoter replacement approach where promoters known to function in the heterologous host are inserted into the biosynthetic gene cluster. The second is an approach that relies on the heterologous host recognizing and utilizing promoters native to the gene cluster. Both have been successful methodologies and have different strengths and weaknesses, which are highlighted and discussed.

Graphical abstract: The role of transcription in heterologous expression of polyketides in bacterial hosts

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Article information


Submitted
29 Jun 2013
First published
24 Sep 2013

Nat. Prod. Rep., 2013,30, 1391-1411
Article type
Review Article

The role of transcription in heterologous expression of polyketides in bacterial hosts

D. C. Stevens, T. P. A. Hari and C. N. Boddy, Nat. Prod. Rep., 2013, 30, 1391
DOI: 10.1039/C3NP70060G

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